- Clone
- Poly5400 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Isotype
- Goat Polyclonal IgG
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Recombinant human IFN-γ (Cat. No. 570206) (black circles) induces cytotoxicity with recombinant human TNF-α (Cat. No. 570106) on HT-29 cells. LEAF™ purified anti-human IFN-γ antibody (Poly5400) (purple squares) reverses the effect of recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg /mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 1-5 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 540003 | 100 µg | $440.00 | |||
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human IFN-γ
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the cytotoxicity induced by recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg/mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells. ND50 range: 1-5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2904434 (BioLegend Cat. No. 540003)
Antigen Details
- Structure
- Cytokine; dimer
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Antigen References
-
- Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
- De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
- Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
- Gray P, et al. 1987. Lymphokines 13:151.
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Other Formats
View All IFN-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| LEAF™ Purified anti-human IFN-γ | Poly5400 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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LEAF™ Purified anti-human IFN-γ
Recombinant human IFN-γ (Cat. No. 570206) (black circles) in...