- Clone
- Poly5174 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
- Isotype
- Goat Polyclonal IgG
-
Recombinant human IL-1β (Cat. No. 579402) (black circles) induces proliferation of D10.G4.1 cell. LEAF™ purified anti-human IL-1β (clone Poly5174) (purple squares) neutralizes the D10.G4.1 cell proliferation induced by recombinant human IL-1β at 0.03 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have this effect. ND50 range: 2-18 ng/mL. -
Whole cell extracts (15 µg total protein) from indicated cell lines treated with Brefeldin A (Cat. No. 420601) with or without lipopolysaccharide (100 ng/mL) were resolved in 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL LEAF™ purified anti-human IL-1β (clone Poly5174) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-goat IgG. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 517405 | 100 µg | $445.00 | |||
IL-1β is a proinflammatory cytokine essential for host responses and resistance to pathogens, including induction of fever, cell proliferation, differentiation, and apoptosis. IL-1β in humans and mice does not encode a typical signal peptide and, as a result, newly synthesized pro-IL-1β accumulates within the cytoplasm of activated monocytes and macrophages. Conversion of the inactive pro-IL-1β to its mature form requires the proteolytic action of IL-1β-converting enzyme (ICE), also termed caspase-1. Secretion of mature IL-1β from LPS-activated monocytes/macrophages is not a constitutive process. These cells must encounter a secondary stimulus that specifically activates the posttranslational processing events. Moreover, owing to its pro-inflammatory nature, IL-1β is regarded as a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis have been observed under the influence of IL-1β. IL-1β is able to facilitate tumor progression in murine models of lung cancer. In addition, upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human IL-1β
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant human IL-1β at 0.03 ng/mL in D10.G4.1 cells. ND50: 2-18 ng/mL. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Detection: The biotinylated Poly5174 is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601) as the capture antibody and recombinant human IL-1β (Cat. No. 579409) as the standard.
Note: For testing human IL-1β in serum or plasma, BioLegend's LEGEND MAX™ Kits (Cat. No. 437007 & 437008) are specially developed and recommended. - RRID
-
AB_3083208 (BioLegend Cat. No. 517405)
Antigen Details
- Structure
- Cytokines/Chemokines
- Distribution
-
IL-1β is expressed in activated monocytes/macrophages.
- Function
- Interleukin (IL-1β) is a cytokine with a key role in the pathophysiology of local and systemic inflammation. IL-1β induces cytokine, chemokine, proinflammatory molecule secretion, and adhesion molecule expression in diverse cells.
- Interaction
- Hematopoietic stem and progenitors
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Stevenson FT, et al. 1992. J Cell Physiol. 152:223-31.
- Shi J, et al. 2007. J. Immunol. 179:1245--53
- Dinarello CA. 2006. Cancer Metastasis Rev. 25:307-13.
- Boost KM, et al. 2008. BMC Cancer. 8:265.
- Gene ID
- 3553 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org
Other Formats
View All IL-1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IL-1β | Poly5174 | ELISA Detection |
| LEAF™ Purified anti-human IL-1β | Poly5174 | Neut,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-human IL-1β
-
LEAF™ Purified anti-human IL-1β
Recombinant human IL-1β (Cat. No. 579402) (black circles) in... 
Whole cell extracts (15 µg total protein) from indicated cel...