- Clone
- Poly5233 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leukemia Inhibitory Factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
- Isotype
- Goat Polyclonal IgG
-
Recombinant human LIF (Cat. No. 593904) (black circles) induces proliferation on human TF-1 cells. Ultra-LEAF™ purified anti-human LIF antibody (Poly5223) (purple squares) neutralizes the cell proliferation induced by recombinant human LIF at 3 ng/mL in human TF-1 cells in a dose-dependent manner whereas the GoInVivo™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.04 - 0.2 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 523303 | 100 µg | $417.00 | |||
Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF displays diverse biological effects but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulation of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively, spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF can be upregulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human LIF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of proliferation induced by recombinant human LIF (Cat. No. 593904) at 3 ng/mL on human TF-1 cells. ND50 range: 0.04 - 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application
- Application Notes
-
ELISA Capture: To measure human LIF, this antibody can be used as a capture antibody in a sandwich ELISA format and paired with the biotinylated clone M017C3 antibody as the detection antibody. Recombinant Human LIF (ELISA Std.) can be used as the protein standard.
Note: For testing human LIF in serum, plasma, or supernatant, BioLegend’s LEGEND MAX™ Human LIF ELISA Kit is specially developed and recommended. - RRID
-
AB_2892478 (BioLegend Cat. No. 523303)
Antigen Details
- Structure
- Four-helix bundle fold.
- Distribution
-
Expressed in the trophectoderm of the developing embryo and activated CD4+ T cells.
- Function
- Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNFα and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
- Interaction
- Embryonic stem cells.
- Ligand/Receptor
- LIFR complex (gp190 and gp130)
- Cell Type
- T cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157. - Gene ID
- 3976 View all products for this Gene ID
- UniProt
- View information about LIF on UniProt.org
Other Formats
View All LIF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LIF | Poly5233 | ELISA Capture,WB |
| LEAF™ Purified anti-human LIF | Poly5233 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human LIF
Whole cell extracts (15 µg total protein) from Daudi cells, ... -
LEAF™ Purified anti-human LIF
Recombinant human LIF (Cat. No. 593904) (black circles) indu...