- Clone
- Poly5355 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Platelet-Derived Growth Factor-AA, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF
- Isotype
- Goat Polyclonal IgG
-
Anti-human PDGF-AA inhibits NIH 3T3 cell proliferation induced by 300 ng/mL recombinant human PDGF-AA (Cat. No. 773702). The ND50 for this effect is 2-10 µg/mL. -
IHC staining of purified anti-human PDGF-AA (clone Poly5355) on formalin-fixed paraffin-embedded human breast cancer (A, positive control) and breast (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of purified anti-human PDGF-AA overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) and HRP anti-goat antibody were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm -
Total cell lysates (15 µg total protein) from Jurkat (negative control, Lane 1) and Jurkat lysate spiked with various amounts of recombinant human PDGF-AA protein (Cat. No. 773702) at 100 ng (positive control, Lane 2), 50 ng (positive control, Lane 3), and 10 ng (positive control, Lane 4) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of purified anti-human PDGF-AA overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-goat IgG. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight ladder
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 535503 | 100 µg | $440.00 | |||
Platelet-derived growth factors (PDGFs) are homo- or heterodimers of four polypeptides, known as A, B, C and D chains. PDGFs usually act primarily in paracrine manner and may be engaged in autocrine loops in tumors. Five PDGF isoforms (AA, BB, AB, CC and DD) have been identified. PDGFs are primarily synthesized, stored, and released by platelets upon activation. PDGF-A and PDGF-B undergo intracellular activation during transport in the exocytic pathway and PDGF-C and PDGF-B are activated by extracellular proteases. Like PDGF-B, intracellular activation of PDGF-A, by removing the N-terminal sequence, is required for PDGF-A to bind to its receptor. PDGF-A can also be expressed by epithelial cells, smooth muscle cells, and neuronal progenitors. PDGF-AA is well known as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). PDGF-AA can promote embryonic forebrain neural stem cells into O4-positive oligodendrocytes by activating Erk pathway. PDGF-AA also plays an important role in directing cell migration during embryonic development and postnatal migration response including wound healing. Chemotaxis ability of PDGF-AA has been shown on fibroblasts, smooth muscle cells, monocytes, and granulocytes.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human PDGF-AA
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
IHC-P, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of NIH 3T3 cell proliferation induced by recombinant human PDGF-AA (Cat. No. 773702) at 300 ng/mL. The ND50 is 2 – 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910467 (BioLegend Cat. No. 535503)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Platelets produce PDGF-AA. Other cells, including epithelial cells, smooth muscle cells, and neuronal progenitors, can also produce PDGF-AA.
- Function
- PDGF-A has two isoforms due to alternative splicing which is cell type-specific and is different among organs and tumor cell lines. Expression of PDGF-A can be increased in human uterine smooth muscle cells during the physiological hypertrophy. Expression of PDGF-A can be repressed by glucocorticoid treatment of smooth muscle cells and aging of human fibroblasts.
- Interaction
- Smooth muscle cells, connective tissue cells, bone and cartilage cells, and oligodendrocyte cells
- Ligand/Receptor
- PDGFR-α
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Heldin CH and Westermark B. 1999. Physiol Rev. 79:1283-316.
- Fredriksson L, et al. 2004. Cytokine Growth Factor Rev. 15:197-204.
- Hu JG, et al. 2012. J Mol Neurosci. 46:644-53.
- Schneider L, et al. 2010. Cell Physiol Biochem. 25:279-92.
- Karlsson C and Paulsson Y. 1994. J Cell Physiol. 158:256-62.
- Shure D, et al. 1992. Biochem Biophys Res Commun. 186:1510-4.
- Gene ID
- 5154 View all products for this Gene ID
- UniProt
- View information about PDGF-AA on UniProt.org
Other Formats
View All PDGF-AA Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| LEAF™ Purified anti-human PDGF-AA | Poly5355 | Neut,IHC-P,WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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