- Clone
- Poly5232 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
- Isotype
- Goat Polyclonal IgG
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Recombinant mouse LIF (Cat. No. 554002) (black circles) inhibits proliferation of M1 cells. LEAF™ purified anti-mouse LIF (clone Poly5232) (purple squares) neutralizes the cell proliferation inhibited by recombinant mouse LIF at 0.5 ng/mL on M1 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG, isotype control (gray triangles) does not have the effect. ND50 range: 0.004 – 0.028 µg/mL -
Whole cell extracts (15 µg total protein) from Raw264.7 cells spiked with the indicated amount of recombinant mouse LIF protein (Cat. No. 554008) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL LEAF™ purified anti-mouse LIF (clone Poly5232) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 523205 | 100 µg | $400.00 | |||
Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant Mouse LIF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of proliferation inhibited by recombinant mouse LIF (Cat. No. 554002) at 0.5 ng/mL on M1 cells. ND50 range: 0.004 – 0.028 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2941596 (BioLegend Cat. No. 523205)
Antigen Details
- Structure
- Monomer; mouse LIF is a 203 amino acid protein with a predicted molecular weight of approximately 22 kD.
- Distribution
-
Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells
- Function
- Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
- Interaction
- Embryonic stem cells
- Ligand/Receptor
- LIFR
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Moreau JF, et al. 1988. Nature. 336:690-2.
- Tomida M, et al. 1993. FEBS Lett. 334:193-7.
- Metcalfe SM. 2011. Genes Immun. 12:157-68.
- Voyle RB, et al. 1999. Exp Cell Res. 249:199-211.
- Slaets H, et al. 2010. Trends Mol Med. 16:493-500.
- Gene ID
- 16878 View all products for this Gene ID
- UniProt
- View information about LIF on UniProt.org
Other Formats
View All LIF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse LIF | Poly5232 | ELISA Capture |
| Biotin anti-mouse LIF | Poly5232 | ELISA Detection |
| LEAF™ Purified anti-mouse LIF | Poly5232 | Neut,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse LIF
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Biotin anti-mouse LIF
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LEAF™ Purified anti-mouse LIF
Recombinant mouse LIF (Cat. No. 554002) (black circles) inhi... 
Whole cell extracts (15 µg total protein) from Raw264.7 cell...