- Clone
- Poly23A06 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-13
- Isotype
- Goat Polyclonal IgG
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Recombinant rat IL-13 (Cat. No. 751702) (black circles) induces proliferation of TF-1 cells. LEAF™ purified anti-rat IL-13 (clone Poly23A06) (purple squares) neutralizes the TF-1 cell proliferation induced by recombinant rat IL-13 at 7 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.04-0.24 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 532604 | 100 µg | $470.00 | |||
Rat IL-13 was initially cloned from the rat renal cortex RNA after the induction of anti-glomerular basement membrane antibody-induced glomerulonephritis. Rat IL-13 respectively shows 63% and 79% homology with the human and mouse proteins at the amino acid level. IL-13 is an immunoregulatory cytokine secreted predominantly by activated Th2 cells, and is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit: the alpha subunit of the IL-4 receptor (IL-4Rα). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins: IL-13Rα1 and IL-13Rα2. The ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13, like IL-4, is a cytokine produced by Th2 cells in response to the signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, chronic obstructive pulmonary disease (COPD), inflammatory bowel disease, and parasitic nematode expulsion.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant Rat IL-13
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant rat IL-13 (Cat. No. 751702) at 7 ng/mL in TF-1 cells. ND50: 0.04 - 0.24 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Monomer
- Distribution
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IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils, natural killer cells (NK), and macrophages.
- Function
- IL-13 modulates human monocytes, macrophages, and B cells. IL-13 induces an increase of MHC class II expression in human monocytes and macrophages, and a direct inhibition of inflammatory cytokines such as TNF-α and IL-1β
- Interaction
- IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells.
- Ligand/Receptor
- IL-4α, IL-13Rα1, and IL-13Rα2
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Lakkis FG and Cruet EN. 1993. Biochem Biophys Res Commun. 197:612-8.
- Papasavvas E, et al. 2005. J Immunol. 175:5532-40.
- Kelly-Welch A, et al. 2005. Sci STKE. 293:cm8.
- Hershey GK. 2003. J Allergy Clin Immunol. 111:677-90.
- Harris J, et al. 2007. Immunity. 27:505-17.
- LaPorte SL, et al. 2008. Cell. 132:259-72.
- Brightling CE,et al. 2010. Clin Exp Allergy. 40:42-9.
- De Paiva CS, et al. 2011. Mucosal Immunol. 4:397-408.
- Gene ID
- 116553 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
Other Formats
View All IL-13 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| LEAF™ Purified anti-rat IL-13 | Poly23A06 | Neut |
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LEAF™ Purified anti-rat IL-13
Recombinant rat IL-13 (Cat. No. 751702) (black circles) indu...