- Clone
- Poly21A06 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte-Colony Stimulating Factor, CSF3, CSF-beta
- Isotype
- Goat Polyclonal IgG
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Recombinant human G-CSF (Cat. No. 578604) (black circles) induces proliferation of M-NFS-60 cells. LEAF™ purified anti-human G-CSF (clone Poly21A06) (purple squares) neutralizes M-NFS-60 cell proliferation induced by recombinant human G-CSF at 0.6 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.02 - 0.1 µg/mL. -
Whole cell extracts (15 µg total protein) were prepared from A431 cells spiked with or without 20 ng of recombinant human G-CSF protein (Cat. No. 578602). Proteins were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 0.5 µg/mL of LEAF™ purified anti-human G-CSF (clone Poly21A06) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat IgG (H+L) antibody at a 1:10,000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 521603 | 100 µg | $421.00 | |||
Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human G-CSF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
-
Neut - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant human G-CSF (Cat. No. 578604) at 0.6 ng/mL on M-NFS-60 cells. ND50 range: 0.02 - 0.1 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody has not been tested for ICC.
- RRID
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AB_2927858 (BioLegend Cat. No. 521603)
Antigen Details
- Structure
- Heterodimer
- Distribution
-
Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells
- Function
- G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
- Interaction
- Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell lines
- Ligand/Receptor
- CD114 (G-CSFR)
- Antigen References
-
- Nagata S, et al. 1986. Nature. 319:415.
- Kim HK, et al. 2006. Blood. 108:812.
- Donahue RE, et al. 2009. Blood. 114:2530.
- Shimoji K, et al. 2010. Cell Stem Cell. 6:227.
- Martins A, et al. 2010. IUBMB Life. 62:611.
- Hara M, et al. 2011. J Exp Med. 208:715.
- Gene ID
- 1441 View all products for this Gene ID
- UniProt
- View information about G-CSF on UniProt.org
Other Formats
View All G-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| LEAF™ Purified anti-human G-CSF | Poly21A06 | Neut,WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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LEAF™ Purified anti-human G-CSF
Recombinant human G-CSF (Cat. No. 578604) (black circles) in... 
Whole cell extracts (15 µg total protein) were prepared from...