- Regulatory Status
- RUO
- Other Names
- Free Active TGF-β1 Pre-coated ELISA Kit
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 437707 | 1 Pre-coated Plate | $497.00 | |||
Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF-β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region (latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF-β1 upon activation.
Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β1 complex (SLC, 100 kD). The large latent TGF-β1 Complex (LLC, 235 - 260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond. The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites. Free active TGF-β1 can be released (activated) by many factors including enzymes and low or high pH.
TGF-β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis, etc.
It is expected that normal serum, plasma, or other biological fluid contains low concentration of free active TGF-β1 and high concentration of Latent TGF-β1. It is the free active form TGF-β1 that binds TGF-β receptor and exerts biological functions. However, it has been difficult to quantify the free active TGF-β1 because of insufficient sensitivities of most assay products currently available on the market. It is necessary to measure both the free active form and total TGF-β1 in biological samples to understand the TGF-β1 functions.
Kit Contents
- Kit Contents
-
- Anti-TGF-β1 Pre-coated 96-well Strip microplate
- TGF-β1 Detection Antibody
- TGF-β1 Standard
- Avidin-HRP D
- Assay Buffer C
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Other species
- Application
-
ELISA
- Additional Product Notes
-
View more applications data for this product in our Scientific Poster Library.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- Sensitivity
- 2.3 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- Many cell types, highly expressed on activated Tregs and platelets
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 21803 View all products for this Gene ID
- UniProt
- View information about TGF-beta1 on UniProt.org