LEGEND MAX™ High Sensitivity Human IFN-γ ELISA Kit

Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine that is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids, including a secretory signal sequence of 23 amino acids. The protein is glycosylated.

The BioLegend LEGEND MAX™ High Sensitivity Human IFN-γ ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human IFN-γ antibody. The detection antibody is a biotinylated mouse monoclonal anti-human IFN-γ antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IFN-γ. This kit is specifically designed for the accurate quantitation of human IFN-γ from serum, heparin plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.