LEGEND MAX™ High Sensitivity Human IL-6 ELISA Kit

IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, and CTL differentiation factor, etc.) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth and it induces nerve cells differentiation. In hepatocytes, it induces acute phase reactants. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis. 

The BioLegend LEGEND MAX™ High Sensitivity Human IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with rat monoclonal anti-human IL-6 antibody. The detection antibody is a biotinylated rat monoclonal anti-human IL-6 antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IL-6. This kit is specifically designed for the accurate quantitation of IL-6 in serum, plasma, cell culture supernatant, and urine. This kit is analytically validated with ready-to-use reagents.