LEGEND MAX™ High Sensitivity Human TNF-α ELISA Kit

Human Tumor Necrosis Factor α (TNF-α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine that can exert regulatory, inflammatory, and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, NK cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble protein form derives from the membrane form by way of proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane occurs by binding to soluble TNFRSF1A/ TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer.

The BioLegend LEGEND MAX™ High Sensitivity Human TNF-α ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human TNF-α capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human TNF-α antibody. This kit is specifically designed for the accurate quantitation of human TNF-α in serum, plasma, cell culture supernatant, and urine. This kit is analytically validated with ready-to-use reagents.