- Regulatory Status
- RUO
- Other Names
- IFNG, IFG, IFI, interferon gamma, Immune interferon
Cat # | Size | Price | Quantity Check Availability | ||
---|---|---|---|---|---|
430107 | 1 Pre-coated Plate | $409.00 |
Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids including a secretory signal sequence of 23 amino acids. The protein is glycosylated.
BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IFN-γ specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is specifically designed for the accurate quantitation of Human IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
Kit Contents
- Kit Contents
-
- Anti-Human IFN-γ Pre-coated 96-well Strip Microplate
- Human IFN-γ Detection Antibody
- Human IFN-γ Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- Sensitivity
- 5.6 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Biology Area
- Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org