LEGEND MAX™ Human IFN-γ ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IFNG, IFG, IFI, interferon gamma, Immune interferon
human ifn-g_121709
  • human ifn-g_121709
Cat # Size Price Quantity Check Availability
430107 1 Pre-coated Plate $409.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids including a secretory signal sequence of 23 amino acids. The protein is glycosylated.

BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IFN-γ specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is specifically designed for the accurate quantitation of Human IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IFN-γ Pre-coated 96-well Strip Microplate
  • Human IFN-γ Detection Antibody
  • Human IFN-γ Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
  2. Johnston LR, et al. 2015. Atherosclerosis. 239:283. PubMed
Product Citations
  1. Henault J, et al. 2015. Nat Immunol. 17: 196-203. PubMed
  2. Liu T, et al. 2021. Gut. 70:1965. PubMed
  3. Cavers A, et al. 2022. Ann Rheum Dis. 81:1603. PubMed
  4. Zhou Z, et al. 2022. Front Pharmacol. 13:870178. PubMed
  5. Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed
  6. Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
  7. Weist MR, et al. 2018. J Nucl Med. 59:1531. PubMed
  8. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
  9. Johnston L, et al. 2015. Atherosclerosis. 239:283. PubMed
  10. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  11. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
  12. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  13. Zeng Y, et al. 2021. Aging (Albany NY). 13:. PubMed
  14. Do P, et al. 2020. ACS Comb Sci. 533:22. PubMed
  15. Damodharan SN, et al. 2020. Cytotherapy. 22:450. PubMed
  16. Brown CE, et al. 2018. Mol Ther. 26:31. PubMed
Sensitivity
5.6 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 3    Revision Date: 09/13/2022

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account