LEGEND MAX™ Human IL-10 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-10 Pre-coated ELISA Kit
human il-10_121609
  • human il-10_121609
Cat # Size Price Quantity Check Availability
430607 1 Pre-coated Plate $409.00
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Description

IL-10 (Interleukin 10, B cell derived T cell growth factor, B-TCGF, Cytokine synthesis inhibitory factor CSIF, T-cell growth inhibitory factor, TGIF) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B (in human cells). IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI that allows the virus to supress the immune repsonse. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response.

BioLegend’s LEGEND MAX™ Human IL-10 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-10 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-10 ELISA Kit is specifically designed for the accurate quantitation of Human IL-10 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IL-10 Pre-coated 96-well Strip Microplate
  • Human IL-10 Dectection Antibody
  • Human IL-10 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Zeng Y, et al. 2021. Aging (Albany NY). 13:. PubMed
  3. Holtkamp C, et al. 2019. PLoS One. 14:e0217269. PubMed
  4. Cavalcante RS, et al. 2021. Br J Pharmacol. 178:2284. PubMed
Sensitivity
2 pg/mL
Standard Range
3.9-250 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 2    Revision Date: 09/11/2013

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