- Regulatory Status
- RUO
- Other Names
- IL-15 Pre-coated ELISA Kit
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 435107 | 1 Pre-coated Plate | $409.00 | |||
IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils and induces the production of proinflammatory
cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 shares with IL-2 the receptor ß-chain and ?-chain. The IL-15 receptor complex also includes a specific a subunit (IL-15Ra), distinct from the IL-2Ra chain.
BioLegend’s LEGEND MAX™ Human IL-15 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-15 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IL-15 ELISA Kit is specifically designed for the accurate quantitation of Human IL-15 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
Kit Contents
- Kit Contents
-
- Anti-Human IL-15 Pre-coated 96-well Strip Microplate
- Human IL-15 Dectection Antibody
- Human IL-15 Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution E
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 2 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- Dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes.
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3600 View all products for this Gene ID
- UniProt
- View information about IL-15 on UniProt.org