- Regulatory Status
- RUO
- Other Names
- IL-1β Pre-coated ELISA Kit, Human IL-1β Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFN-β-inducing factor, Interleukin-β, Osteoclast activating factor (OAF),
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 437007 | 1 Pre-coated Plate | $409.00 | |||
IL-1 refers to two proteins, IL-1a and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. IL-1β is expressed in several cell types, including monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, and natural killer cells. IL-1β is upregulated by TNF-a, IFN-a, IFN-β, IFN-?, bacterial endotoxins, viruses, mitogens, and antigens; while IL-1β is downregulated by IL-6, lipoproteins, lipids, and a2-macroglobulin. IL-1β plays a role in many physiological and pathological conditions including rheumatoid arthritis and cancer.
BioLegend’s LEGEND MAX™ Human IL-1β ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-1β specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IL-1β ELISA Kit is specifically designed for the accurate quantitation of Human IL-1β from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
Kit Contents
- Kit Contents
-
- Anti-Human IL-1β Pre-coated 96-well Strip Microplate
- Human IL-1β Dectection Antibody
- Human IL-1β Standard
- Streptavidin-Polymer HRP
- Assay Buffer D
- Matrix E (for serum and plasma samples only)
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Application Notes
-
BioLegend's mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.
- Product Citations
-
- Sensitivity
- 0.5 pg/mL
- Standard Range
- 2.0-125 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3553 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org