LEGEND MAX™ Human IL-8 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-8 Pre-coated ELISA Kit
human il-8_121609
  • human il-8_121609
Cat # Size Price Quantity Check Availability
431507 1 Pre-coated Plate $409.00
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Description

Human IL-8 (CXCL8, neutrophil chemotactic factor, neutrophil activating protein, monocytes derived neutrophil chemotactic factor) is a member of the alpha (C-X-C) subfamily of chemokines. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation leading to local inflammation in damaged or infected tissues.

BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-8 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is specifically designed for the accurate quantitation of Human IL-8 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IL-8 Pre-coated 96-well Strip Microplate
  • Human IL-8 Dectection Antibody
  • Human IL-8 Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Shi JX, et al. 2012. Am J Physiol Cell Mol Physiol. 302:793. PubMed
  2. Kummaraputugu AB, et al. 2013. J. Biol Chem. 288:4681. PubMed
  3. Shi JX, et al. 2014. Cell Signal. 26:2390. PubMed
Product Citations
  1. Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed
  2. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  3. Shi J, et al. 2014. Int Immunopharmacol. 22:480. PubMed
  4. Zhang Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:611. PubMed
  5. Shi J, et al. 2014. Cell Signal. 26:2390. PubMed
  6. Spittaels KJ, et al. 2021. iScience. 24:102575. PubMed
  7. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  8. Shi J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L793. PubMed
  9. Shi JX, et al. 2018. Mol Med Rep. 2.361805556. PubMed
  10. Dudau M, et al. 2021. Front Pharmacol. 12:737571. PubMed
  11. Kummarapurugu A, et al. 2013. J Biol Chem. 288:4861. PubMed
Sensitivity
4.4 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org
Go To Top Version: 2    Revision Date: 09/11/2013

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