LEGEND MAX™ Human Total IgG ELISA Kit

IgG is a monomeric immunoglobulin composed of two identical heavy chains and two identical light chains linked together by inter-chain disulfide bonds. There are four IgG subclasses (IgG1, IgG2, IgG3, and IgG4) in humans named in order of decreasing abundance. They differ in the size of the hinge region and the number and arrangements of the interchain disulfide bonds linking their heavy chains. Of the 5 immunoglobulin isotypes, IgG is most abundant in human serum. IgG is synthesized and secreted by plasma B cells and is involved in the secondary immune response. The measurement of IgG can be a diagnostic tool for certain conditions such as autoimmune diseases, gastrointestinal conditions or immunoglobulin-deficiency disorders. 

The BioLegend LEGEND MAX™ Human Total IgG ELISA Kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a human monoclonal anti-human IgG antibody. The detection antibody is a biotinylated anti-human IgG antibody. This kit is specifically designed for the accurate quantitation of human IgG in serum, plasma, breast milk, saliva and urine. This kit is analytically validated with ready-to-use reagents.