LEGEND MAX™ Human VEGF ELISA Kit

VEGF (also known as VEGF-A) is a signaling protein with strong vascular permeability activity that stimulates the formation of new blood vessels during the processes of vasculogenesis and angiogenesis. It is a member of the vascular endothelial growth factor family, which in mammals is comprised of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). There are multiple isoforms of VEGF that result from alternative splicing of mRNA. VEGF binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), through which VEGF exerts its mitogenic effects. VEGF is highly expressed in solid tumors of breast, lung, renal, colorectal, and hepatic origin. Additionally, it significantly contributes to ascites tumor formation. Beyond cancer, VEGF has also been associated with multiple pathogenic conditions including rheumatoid arthritis, age-related macular degeneration, Crow-Fukase syndrome, sclerosis and Alzheimer’s disease.

The BioLegend LEGEND MAX™ Human VEGF Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human VEGF capture antibody. The detection antibody is a biotinylated goat polyclonal anti-human VEGF antibody. This kit is specifically designed for the accurate quantitation of free-form human VEGF from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.