LEGEND MAX™ Mouse IL-10 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 10 (IL-10), B cell derived T cell growth factor (B-TCGF
IL-10_Mouse_LegMax_080612
  • IL-10_Mouse_LegMax_080612
Cat # Size Price Quantity Check Availability
431417 1 Pre-coated Plate $345.00
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Description

Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.


The BioLegend LEGEND MAX™ Mouse IL-10 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantitation of mouse IL-10 from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.

Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse IL-10 Pre-coated 96-well Strip Microplate
  • Mouse IL-10 Dectection Antibody
  • Mouse IL-10 Standard
  • Matrix C (for serum and plasma samples only)
  • Streptavidin-Polymer HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
  2. McKelvey R, et al. 2015. J Neurosci. 35:457. PubMed
Product Citations
  1. Bai H, et al. 2022. Scand J Immunol. 95:e13158. PubMed
  2. Weng TI, et al. 2019. Contrast Media Mol Imaging. 2018:3535769. PubMed
  3. Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
  4. Lian H, et al. 2021. Nat Microbiol. 6:658. PubMed
  5. Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed
  6. McKelvey R, et al. 2015. J Neurosci. 35:457. PubMed
  7. Kueanjinda P, Palaga S 2015. Sci Rep. 5: 12784. PubMed
  8. Cen X, et al. 2020. Nat Commun. 4.438194444. PubMed
  9. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
Sensitivity
2.7 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 3    Revision Date: 07/05/2024

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