- Regulatory Status
- RUO
- Other Names
- IL-22 Pre-coated ELISA Kit
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Naïve CD4 T cells were harvested from the spleens of Il22+/+ or Il22-/- mice (C57BL/6 background, see Immunity. 2007 Oct; 27(4):647-59).
Cells were then cultured with plate-bound aCD3 and aCD28 mAbs in IMDM (Th0 Conditions); or additionally with recombinant mouse IL-23 (50 ng/ml), IL-1b (20 ng/ml), IL-6 (20 ng/ml) and 200 nM 6-formylindolo(3,2-b)carbazole (Th22 Conditions).
Three days post-stimulation, supernatants were harvested, and IL-22 was quantitated using the LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307).
Data were provided courtesy of Prof. Lauren A. Zenewicz, University of Oklahoma.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 436307 | 1 Pre-coated Plate | $409.00 | |||
IL-22 belongs to a family of cytokines structurally related to IL-10, including IL-19, IL-20, IL-24, and IL-26. Mouse IL-22 shares 79% amino acid identity with human IL-22. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of the T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in the disregulated inflammation observed in autoimmune diseases.
BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-22 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-22 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
Kit Contents
- Kit Contents
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- Anti-Mouse IL-22 Pre-coated 96-well Strip Microplate
- Mouse IL-22 Dectection Antibody
- Mouse IL-22 Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Mouse
- Application
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ELISA
- Product Citations
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- Sensitivity
- 1.6 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
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- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- Th17, Th1, Th2, Th22, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 50929 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org