LEGEND MAX™ Mouse IL-4 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-4 Pre-coated ELISA Kit
IL-4 061709
  • IL-4 061709
Cat # Size Price Quantity Check Availability
431107 1 Pre-coated Plate $345.00
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Description

IL-4 (Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1,BSF-1, Hodgkin’s cell growth factor, HCGF, Mast cell growth factor-2, MCGF-2, Macrophage fusion factor, MFF, T cell growth factor-2, TCGF-2) is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. IL-4 regulates differentiation of naïve CD4+ T cell to Th2 cells. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. In addition, IL-4 regulates IgE and IgG1 production by B cells.

BioLegend’s LEGEND MAX™ Mouse IL-4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-4 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IL-4 ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-4 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse IL-4 Pre-coated 96-well Strip Microplate
  • Mouse IL-4 Dectection Antibody
  • Mouse IL-4 Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Product Citations
  1. Li B et al. 2019. Cell reports. 26(10):2720-2737 . PubMed
Sensitivity
0.5 pg/ml
Standard Range
2-125 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Mast cells, T cells, bone marrow stromal cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16189 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org
Go To Top Version: 2    Revision Date: 09/11/2013

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