Pricing & Availability
Technical data sheet
- Regulatory Status
- RUO
- Other Names
- TNF-a ELISA Kit
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 430907 | 1 Pre-coated Plate | $345.00 | |||
Description
TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-a. TNF-a forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-a has also been described. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Kit Contents
- Kit Contents
-
- Anti-Mouse TNF-α Pre-coated 96-well Strip Microplate
- Mouse TNF-α Dectection Antibody
- Mouse TNF-α Standard
- Matrix A (for serum and plasma samples only)
- Avidin-HRP B
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution D
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- Product Citations
-
- Sensitivity
- 1.5 pg/ml
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Sources
- Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 21926 View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org