- Regulatory Status
- RUO
- Other Names
- TGF-β1 (Free Active Form or Total)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 740450 | 100 tests | $470.00 | |||
The LEGENDplex™ Human Free Active/Total TGF-β1 Assay is a single plex bead-based assay, using fluorescence–encoded beads suitable for use on various flow cytometers. This assay allows quantification of either free active TGF-β1 without sample treatment or total TGF-β1 after sample treatment. It provides high detection sensitivities and broad dynamic range. The assay has been validated for use with serum, plasma, cell culture supernatant, saliva, and urine samples.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- Capture Beads
- Detection Antibodies
- Standard Cocktail, Lyophilized
- Acidification Solution
- Neutralization Solution
- Sample Diluent
- SA-PE
- Matrix B, Lyophilized
- Assay Buffer
- Wash Buffer
- Plate Sealers
- V-bottom Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex.
Download the LEGENDplex™ software here. - Application Notes
-
The assay is able to detect free active TGF-ß1 without sample treatment or total TGF-ß1 after sample treatment. This assay has been fully verified for mouse/rat as well as human samples. Though not specifically tested, there is an expected cross reactivity with multiple species of non-human primates. There is > 99% amino acid identity conservation between the human sequence and that of the Rhesus macaque (M. mulatta), Cynomolgous macaque (M. fascicularis), Chimpanzee (genus Pan), and Baboon (genus Papio); species which comprise the vast majority of primates used in current Biomedical Research.
- Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
Antigen Details
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 7040 View all products for this Gene ID