- Regulatory Status
- RUO
- Other Names
- TGF-β1 (Free Active Form or Total Form)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 740490 | 100 tests | $470.00 | |||
The LEGENDplex™ Mouse/Rat Free Active/Total TGF-β1 Assay is a single plex bead-based assay, using fluorescence–encoded beads suitable for use on various flow cytometers. This assay allows quantification of either free active TGF-β1 without sample treatment or total TGF-β1 after sample treatment. It provides high detection sensitivities and broad dynamic range. The assay has been validated for use with serum, plasma, cell culture supernatant samples.
Kit Contents
- Kit Contents
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- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- Capture Beads
- Detection Antibodies
- Standard Cocktail, Lyophilized
- Acidification Solution
- Neutralization Solution
- Sample Diluent
- SA-PE
- Matrix C1, Lyophilized
- Assay Buffer
- Wash Buffer
- Plate Sealers
- V-bottom Plate
Product Details
- Verified Reactivity
- Mouse, Rat
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex.
Download the LEGENDplex™ software here. - Application Notes
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The assay is able to detect free active TGF-ß1 without sample treatment or total TGF-ß1 after sample treatment. This assay has been fully verified for mouse/rat as well as human samples. Though not specifically tested, there is an expected cross reactivity with multiple species of non-human primates. There is > 99% amino acid identity conservation between the human sequence and that of the Rhesus macaque (M. mulatta), Cynomolgous macaque (M. fascicularis), Chimpanzee (genus Pan), and Baboon (genus Papio); species which comprise the vast majority of primates used in current Biomedical Research.
- Product Citations
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- Materials Not Included
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- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
Antigen Details
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 21803 View all products for this Gene ID