- Regulatory Status
- RUO
-
A single cell suspension from human PBMCs was prepared for the positive selection of CD3+ cells using the MojoSort™ Human CD3 Selection Kit. Cells were stained with anti-human CD3 (clone UCHT1) PE and anti-human CD14 (clone M5E2) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: CD3+ cells after isolation. Right plot: Cells before isolation.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480133 | 20 tests | $249.00 | |||
| 480134 | 200 tests | $723.00 | |||
Human CD3+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-human CD3 antibody and magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD3+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480133:
- 1 vial containing 200 µl of Biotin anti-human CD3 antibody
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat# 480134:- 2 vials containing 1mL each of Biotin anti-human CD3 antibody
- 2 vials containing 1mL each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Particle: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
-
Biotin Antibody: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Conjugated nanobeads: Protein-coated magnetic beads. - Application
- Recommended Usage
-
10 µL of Biotin anti-human CD3 antibody for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer. - Application Notes
-
The expected purity is >80% CD3+/CD14- cells post-separation of human peripheral blood mononuclear cells from healthy donors, analyzed by flow cytometry
This kit is designed for the positive selection or depletion of CD3+ cells from human PBMCs.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 7X
Nanobead dilution to use in columns: 15X
Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD3 on UniProt.org