- Regulatory Status
- RUO
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A single cell suspension from human monocyte depleted peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD4+ cells using the MojoSort™ Human CD4 Nanobeads. Cells were stained with CD4 (clone OKT4) PE and CD3 (clone UCHT1) APC. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480013 | 10 tests | $198.00 | |||
| 480014 | 100 tests | $594.00 | |||
CD4+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD4 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480013:
- 1 vial containing 100 µl CD4 nanobeads
For Cat# 480014:
- 1 vial containing 1 ml of CD4 Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Human CD4 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Human CD4 Nanobeads for 1x107 cells in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive isolation of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs). To maximize the purity of CD4+ T cells, we strongly recommend depletion of monocytes prior to isolation. Efficient isolation of monocytes can be achieved using Biotin anti-human CD14 antibody and Streptavidin Nanobeads.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 5X
To find out the dilution to use this product in separation columns please download the CoA. - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org