- Regulatory Status
- RUO
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A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD11b+ cells using the MojoSort™ Mouse CD11b+ Selection Kit. Left plot: CD11b+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD11b+ (clone M1/70) APC, Ly-6G/Ly-6C (Gr-1) PE. Dead cells were excluded by Helix NP™ Blue. -
A single cell suspension from C57BL/6 mouse brain was prepared for the positive selection of CD11b+ cells using the MojoSort™ Mouse CD11b+ Selection Kit. Right plot: CD11b+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD11b+ (clone M1/70) APC and P2RY12 (clone S16007D) PE. Dead cells were excluded by Helix NP™ Blue.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480109 | 20 tests | $243.00 | |||
| 480110 | 200 tests | $746.00 | |||
Mouse CD11b+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD11b+ antibody and then magnetic streptavidin nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD11b+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat. No. 480109:
- 1 vial containing 200 µL of biotin anti-mouse CD11b+ antibody (clone M1/70)
- 1 vial containing 200 µL of streptavidin nanobeads
For Cat. No. 480110:- 2 vials containing 1 mL each of biotin anti-mouse CD11b+ antibody (clone M1/70)
- 2 vials containing 1 mL each of streptavidin nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
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Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Streptavidin nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
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The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin nanobeads: Protein-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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Volume of biotin antibody and streptavidin nanobeads should be adjusted depending on the different tissue of CD11b+ cells to be isolated. Use the table below as an example when working with different mouse tissue. For 1x107 cells in 100 µL of buffer, use the following volumes:
Tissue
Biotin Antibody Optimal Nanobeads Volume
Mouse Bone Marrow
10 µL
20 µL
Mouse Splenocytes
5 µL
10 µL
Mouse Lung
10 µL
10 µL
Mouse Brain
10 µL
2 µL
The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. Nos. 480109 & 480110). For low frequency cells, pre-dilute the streptavidin nanobeads in order to pipette a minimum of 5 µL of any solution. Avoid working with small volumes. - Application Notes
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This kit is designed for the positive selection or depletion of mouse CD11b+ cells from mouse bone marrow, lymphoid tissue, lung and brain, among other organs.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
This kit is not recommended for CD11b positive selection from human samples based on in-house testing results.
Antibody or cocktail dilution to use in column: 32X
Nanobead dilution to use in columns: 5X - Product Citations
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Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD11b on UniProt.org