- Regulatory Status
- RUO
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A single cell suspension from C57BL/6 mouse splenocytes was prepared for positive selection of CD3+ cells using the MojoSort™ Mouse CD3 selection Kit. Cells were stained with anti-mouse CD3 (clone 145-2C11) PE. Dead cells was excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480099 | 20 tests | $198.00 | |||
| 480100 | 200 tests | $594.00 | |||
Mouse CD3+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse CD3 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD3+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Clone 17A2 recognizes ε/γ (but not ε/δ) of the CD3 complex. It can induce T cell activation.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat. No. 480099:
- 1 vial containing 200 µl of Biotin anti-mouse CD3 antibody (clone 17A2)
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat. No. 480100:
- 2 vials containing 1ml each of Biotin anti-mouse CD3 antibody (clone 17A2)
- 2 vials containing 1ml each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
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Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
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The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin Nanobeads: protein-coated magnetic nanobeads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of Biotin anti-mouse CD3 antibody for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the positive selection or depletion of mouse CD3+ cells from lymphoid tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 4X - Product Citations
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Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Gene ID
- NA
- UniProt
- View information about CD3 on UniProt.org