- Regulatory Status
- RUO
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A single cell suspension from collagenase digested 4T1-induced tumors were depleted of CD45+ cells using CD45 Nanobeads (Cat. No. 480028). CD326+ (Ep-CAM) cells were then isolated using MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD. Left plot: CD45-CD326 (Ep-CAM)+ cells after isolation. Right plot: Cells before isolation. -
A single cell suspension mixture of 90% C57BL/6 mouse splenocyte with 10% 4T1 mouse cell line was prepared for positive selection of CD326+ (Ep-CAM) cells using the MojoSort™ Mouse CD326 (Ep-CAM) selection Kit. Left plot: CD326+ (Ep-CAM) cells after isolation. Right plot: Cells before isolation. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480141 | 20 tests | $219.00 | |||
| 480142 | 100 tests | $430.00 | |||
Mouse CD326 (Ep-CAM) cells are selected by incubating the sample with biotin conjugated anti-mouse CD326 antibody after blocking with TruStain FcX (anti-mouse CD16/32 antibody) then followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD326 (Ep-CAM) expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. In addition to CD326 (Ep-CAM) expression on epithelial and tumor cells, CD326 is also expressed on subtypes of leukocytes, which should be depleted using MojoSort™ Mouse CD45 Nanobeads before CD326 positive selection.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat# 480141:
- 1 vial containing 200 µL of Biotin anti-mouse CD326 antibody (clone G8.8)
- 1 vial containing 200 µL of Streptavidin Nanobeads
- 1 vial containing 200 µL of each TruStain FcX (Anti-mouse CD16/32 antibody)
For Cat# 480142:- 1 vial containing 1mL of each of Biotin anti-mouse CD326 antibody (clone G8.8)
- 1 vial containing 1mL of each of Streptavidin Nanobeads
- 1 vial containing 1mL of each of TruStain FcX (Anti-mouse CD16/32 antibody)
Product Details
- Verified Reactivity
- Mouse
- Formulation
- Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer. Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
- Preparation
- Conjugated nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
- Recommended Usage
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5 µL of TruStain FcX (Anti-mouse CD16/32 antibody) for 1 x 107 cells in 100 µL of buffer.
10 µL of Biotin anti-mouse CD326 antibody for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer. - Application Notes
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This kit is designed for the positive selection of mouse CD326 (Ep-CAM) cells from single-cell preparations of epithelial or tumor cells.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
To find out the dilution to use this product in separation columns please download the CoA.
Antibody or cocktail dilution to use in column: 1.6X
SAV Particle dilution to use in columns: 16X
Antigen Details
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD326 on UniProt.org