- Regulatory Status
- RUO
-
A single cell suspension from C57BL/6 mouse splenocytes was prepared for positive selection of CD90.2+ cells using the MojoSort™ Mouse CD90.2 selection Kit. Cells were stained with anti-mouse CD90.2 (clone 30-H12) APC and CD3 (clone 17A2) FITC. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480101 | 20 tests | $264.00 | |||
| 480102 | 200 tests | $773.00 | |||
Mouse CD90.2+ cells are either selected or depleted by incubating the sample with biotin conjugated anti-mouse CD90.2 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD90.2+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat. No. 480101:
- 1 vial containing 200 µl of Biotin anti-mouse CD90.2 antibody (clone 30-H12)
- 1 vial containing 200 µl Streptavidin Nanobeads
- 1 vial containing 50 µg TruStain FcX™ PLUS anti-mouse CD16/32 antibody
For Cat# 480102:- 2 vials containing 1 ml each of Biotin anti-mouse CD90.2 antibody (clone 30-H12)
- 2 vials containing 1 ml each of Streptavidin Nanobeads
- 1 vial containing 500 µg TruStain FcX™ PLUS anti-mouse CD16/32 antibody
* Older lots for this kit may not contain TruStain FcX™ PLUS component. These kits can be used without the addition of this reagent.
Product Details
- Verified Reactivity
- Mouse
- Formulation
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Cocktail: Phophate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin Nanobeads: protein-coated magnetic nanobeads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
Volume of Streptavidin Nanobeads should be adjusted depending on starting percentage of CD90.2+ cells to be isolated. Use the table below as an example when working with a cell mixture of C57BL/6 (CD90.2+) and FVB/NJ (CD90.2-) mice. For 1x107 cells in 100 µl of buffer, use the following volumes:
C57BL/6 + FVB/NJ Mixture (spleen)
Starting CD90.2 Frequency
Optimal Nanobeads Volume
10% C57BL/6 + 90% FVB/NJ
3.0%
0.77 µl
25% C57BL/6 + 75% FVB/NJ
7.5%
0.77 µl
50% C57/BL/6 + 50% FVB/NJ
15.0%
7.7 µl
75% C57BL/6 + 25% FVB/NJ
22.5%
7.7 µl
100% C57BL/6
30.0%
10 µl
The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. No. 480019/480020). For low frequency cells, pre-dilute the Streptavidin Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate CD90.2+ cells from a mixture of 25% C57BL/6 and 75% FVB/NJ, pre-dilute 10 µl of Streptavidin Nanobeads in 90 µl of MojoSort™ buffer (Cat. No. 480017) and add 7.7 µl of that dilution per sample. Avoid working with small volumes.
- Application Notes
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This kit is designed for the positive selection or depletion of mouse CD90.2+ cells from lymphoid tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 6X
Nanobead dilution to use in columns: 13X
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD90.2 on UniProt.org