- Regulatory Status
- RUO
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A single cell suspension from C57BL/6 bone marrow was prepared for the positive selection of Ly-6G+ cells using the MojoSort™ Mouse Ly-6G Selection Kit. Cells were stained with anti-mouse Ly-6G (clone 1A8) PE and anti-mouse/human CD11b (clone M1/70) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: Ly-6G+ cells after isolation. Right plot: Cells before isolation.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480123 | 20 tests | $255.00 | |||
| 480124 | 200 tests | $758.00 | |||
Mouse Ly-6G+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse Ly-6G antibody and magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the Ly-6G+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat. No. 480123:
- 1 vial containing 200 µl of Biotin anti-mouse Ly-6G antibody (clone 1A8)
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat. No. 480124:- 2 vials containing 1 ml each of Biotin anti-mouse Ly-6G antibody (clone 1A8)
- 2 vials containing 1 ml each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
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Antibody: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Conjugated nanobeads: Protein-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of Biotin anti-mouse Ly-6G antibody for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the positive selection or depletion of mouse Ly-6G+ cells from lymphoid tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 5X
Nanobead dilution to use in columns: 5X - Product Citations
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Antigen Details
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- NA
- UniProt
- View information about Ly-6G on UniProt.org