ELISA MAX™ Deluxe Set Mouse IL-9

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Mouse Interleukin 9 (IL-9) is a 144 amino acid, 16.1 kD member of the TH2 cytokine family. Originally identified as a mast cell growth factor, IL-9 has been identified to promote IL-6 and protease production in mast cells that can contribute to the severity of hypersensitivity disorders. These characteristics, among others, have made IL-9 a molecule of interest in studies of hypersensitivity disorders, such as asthma.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A mouse IL-9 specific rat monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-9 binds to the immobilized capture antibody.  Next, a biotinylated goat polyclonal anti-mouse IL-9 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-9 present in the sample.  Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.