Pacific Blue™ anti-human CD235ab Antibody

Pricing & Availability
Clone
HIR2 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70299
Other Names
Glycophorin A/B, GPA/GPB, GYPA, GYPB
Isotype
Mouse IgG2b, κ
HIR2_PB_111709
Human erythrocytes stained with HIR2 Pacific Blue™
  • HIR2_PB_111709
    Human erythrocytes stained with HIR2 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
306611 25 µg $153.00
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306612 100 µg $323.00
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Description

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.015 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
Product Citations
  1. Bowley TY, et al. 2022. Cancer Res Commun. 2:1436. PubMed
  2. Hoover MY, et al. 2023. Nat Protoc. . PubMed
  3. Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed
  4. Rothe K, et al. 2020. Cell Stem Cell. 27(1):110-124.e9. PubMed
  5. Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed
  6. Ito Y, et al. 2018. Cell. 174:636. PubMed
  7. Ochi K, et al. 2014. Stem Cells Trans Med. 3:792. PubMed
  8. Pauken CM, et al. 2021. Cancers (Basel). 13:. PubMed
RRID
AB_2248153 (BioLegend Cat. No. 306611)
AB_2248153 (BioLegend Cat. No. 306612)

Antigen Details

Structure
Sialoglycoprotein, 20 kD
Distribution

Erythrocytes

Cell Type
Erythrocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235ab on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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