- Clone
- H1b-98 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b)
- Isotype
- Mouse IgG2b, κ
-
LPS-stimulated (6 hours) human peripheral blood monocytes stained with CD14 PE and intracellular stained with H1b-98 Pacific Blue™
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 511709 | 25 µg | $141.00 | |||
| 511710 | 100 µg | $293.00 | |||
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Product Citations
-
- RRID
-
AB_2124351 (BioLegend Cat. No. 511709)
AB_2124351 (BioLegend Cat. No. 511710)
Antigen Details
- Structure
- Cytokine; 17.3 kD (Mammalian)
- Bioactivity
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
- Cell Targets
- B cells, T cells, monocytes
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095. - Regulation
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 3553 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org
Other Formats
View All IL-1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IL-1β | H1b-98 | ELISA Detection,ELISPOT Detection,ICC |
| FITC anti-human IL-1β | H1b-98 | ICFC |
| Alexa Fluor® 647 anti-human IL-1β | H1b-98 | ICFC |
| Pacific Blue™ anti-human IL-1β | H1b-98 | ICFC |
| TotalSeq™-B1166 anti-human IL-1β | H1b-98 | ICPG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human IL-1β
Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and trea... -
FITC anti-human IL-1β
LPS-stimulated peripheral blood mononuclear cells intracellu... -
Alexa Fluor® 647 anti-human IL-1β
-
Pacific Blue™ anti-human IL-1β
LPS-stimulated (6 hours) human peripheral blood monocytes st... -
TotalSeq™-B1166 anti-human IL-1β