Pacific Blue™ anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-4 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
RM4-4_PB_031512
C57BL/6 mouse splenocytes were stained with CD4 (clone RM4-4) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
  • RM4-4_PB_031512
    C57BL/6 mouse splenocytes were stained with CD4 (clone RM4-4) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
116007 25 µg $101.00
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116008 100 µg $229.00
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Description

CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed
  2. Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
  3. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  4. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  5. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  6. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  7. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  8. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  9. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  10. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  11. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
  12. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
RRID
AB_11147758 (BioLegend Cat. No. 116007)
AB_11147758 (BioLegend Cat. No. 116008)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 2    Revision Date: 01/02/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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