Pacific Blue™ anti-mouse TCR Vβ5.1, 5.2 Antibody

Pricing & Availability
Clone
MR9-4 (See other available formats)
Regulatory Status
RUO
Other Names
Vβ5 T-cell receptor, T cell receptor beta 5
Isotype
Mouse IgG1, κ
MR9-4_PB_TCR_VB_Antibody_071718
C57BL/6 splenocytes were stained with CD3ε PE and TCR Vβ5.1, 5.2 (clone MR9-4) Pacific Blue™ (left) or Mouse IgG1, κ Pacific Blue™ isotype control (right).
  • MR9-4_PB_TCR_VB_Antibody_071718
    C57BL/6 splenocytes were stained with CD3ε PE and TCR Vβ5.1, 5.2 (clone MR9-4) Pacific Blue™ (left) or Mouse IgG1, κ Pacific Blue™ isotype control (right).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
139515 25 µg $101.00
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139516 100 µg $229.00
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Description

Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Murine T cell hybridoma 2HB51.8
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
  2. Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
  3. Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
  4. Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
Product Citations
  1. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  2. Guo R, et al. 2020. Cell Res. 30:21. PubMed
  3. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  4. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  5. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
RRID
AB_2750134 (BioLegend Cat. No. 139515)
AB_2750134 (BioLegend Cat. No. 139516)

Antigen Details

Structure
Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution

Expressed on a subset of TCR αβ+ T cells.

Function
Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor
MHC-Peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171. 
2. Sim GK and Augustin AA. 1985. Cell 42:89. 
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta5.1 5.2 on UniProt.org
Go To Top Version: 1    Revision Date: 07/17/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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