PE anti-γ-Catenin Antibody

Pricing & Availability
Clone
15F11 (See other available formats)
Regulatory Status
RUO
Other Names
Junction Plakoglobin, DP3, Catenin (Cadherin-associated Protein), Gamma 80kDa, Desmoplakin III, CTNNG, PDGB, PKGB, Catenin Gamma
Isotype
Mouse IgG1, κ
15F11_PE_gamma-Catenin_Antibody_102522
MCF7 cells (positive control) (filled histogram) and MOLT-4 cells (negative control) (open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-γ Catenin (clone 15F11), or PE mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 981804).
  • 15F11_PE_gamma-Catenin_Antibody_102522
    MCF7 cells (positive control) (filled histogram) and MOLT-4 cells (negative control) (open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-γ Catenin (clone 15F11), or PE mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 981804).
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946405 25 tests $194.00
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946406 100 tests $382.00
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Description

γ-catenin, also referred to as JUP and plakoglobin, is a member of the Armadillo family of proteins that are required for Wnt signaling. γ-catenin was originally described as a structural component of desmosomes and adherens junctions, where it links E-cadherins to the actin cytoskeleton. The presence of this complex is critical for cell migration and adhesion. While γ-catenin shows high overall similarity to the homolologous β-catenin, notably in the armadillo domain rich central region, γ-catenin has distinct N- and C-terminal regions, which allows interaction with protein partners functionally distinct with those of other catenins. Additionally, γ-catenin shows tumor suppressor activity by downregulation of oncogenic Wnt signaling. This negative regulation occurs through multiple mechanisms: a. displacement of β-catenin from junctional complexes thereby promoting its ubiquitin-dependent degradation, b. disrupting formation of the TCF- β-catenin transcriptional activator complex by directly binding β-catenin, c. stimulating the nuclear export of the SOX4 transcription factor. γ-catenin also plays a role in maintaining embryonic stem cells in a pluripotent state.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant chicken γ-catenin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone was tested for ICC on MCF7 (positive control) and MOLT-4 (negative control) cells using multiple fixation and permeabilization conditions. A brief summary of these results is listed below:

  • methanol only: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Moderate nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus methanol: 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
RRID
AB_2927950 (BioLegend Cat. No. 946405)
AB_2927950 (BioLegend Cat. No. 946406)

Antigen Details

Structure
γ-catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD.
Distribution

Ubiquitously expressed/Cytoskeleton and plasma membrane associated

Function
Cell to cell adhesion, cell signaling
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
Antigen References
  1. Zhurinksy J, et al. 2000. J Cell Sci. 18:3127.
  2. Mahendram S, et al. 2013. PLoS ONE. 8:e65320.
  3. Aktary Z, et al. 2017. Oncotarget. 8:32270.
Gene ID
3728 View all products for this Gene ID
UniProt
View information about gamma-Catenin on UniProt.org
Go To Top Version: 1    Revision Date: 10/13/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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