- Clone
- QA18A19 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- c-kit, stem cell factor receptor, mast cell growth factor receptor, steel factor receptor
- Isotype
- Mouse IgG1, κ
-
Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with anti-human CD117 (c-kit) recombinant (clone QA18A19) PE (closed histogram) or mouse IgG1, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 332203 | 25 tests | $111.00 | |||
| 332204 | 100 tests | $247.00 | |||
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone QA18A19 antibody can completely block the binding of clone 104D2 on target cells.
- RRID
-
AB_2936692 (BioLegend Cat. No. 332203)
AB_2936692 (BioLegend Cat. No. 332204)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Cell Type
- Hematopoietic stem and progenitors, Leukemia, Mast cells
- Biology Area
- Signal Transduction, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Giebel LB, et al. 1992. Oncogene. 7:2207-17.
- Furitsu T, et al. 1993. J Clin Invest. 92:1736-44.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD117 (c-kit) Recombinant Antibody | QA18A19 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD117 (c-kit) Recombinant Antibody
Kasumi-3 cells (an acute myeloblastic leukemia cell line) we...