PE anti-human CD119 (IFN-γ R α chain) Antibody

Pricing & Availability
Clone
GIR-94 (See other available formats)
Regulatory Status
RUO
Other Names
IFN-γR, IFN-γRα, IFN-gamma R1, CDw119
Isotype
Mouse IgG2b, κ
GIR-94
Human peripheral blood lymphocytes stained with biotinylated GIR-94 and detected with Sav-PE
  • GIR-94
    Human peripheral blood lymphocytes stained with biotinylated GIR-94 and detected with Sav-PE
See PE spectral data
Cat # Size Price Quantity Check Availability
308703 25 tests $110.00
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308704 100 tests $244.00
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Description

CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IFN-γRα, purified from human placenta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting3,4, immunoprecipitation3,4, immunofluorescence microscopy6. GIR-94 antibody does not inhibit IFN-? binding to IFN-? R a chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308704) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308706) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan K, et al. 1988. J. Immunol. 140:4231.
  2. Jouanguy E, et al. 2000. J. Clin. Invest. 105:1429.
  3. Kaplan D, et al. 1996. J. Biol. Chem. 271:9. (WB IP)
  4. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (WB IP)
  5. Choi JC, et al. 2007. J. Immunol. 178:1598.
  6. Humlicek AL, et al. 2007. J. Immunol. 178:6395. (IF)
  7. Aomatsu M, et al. 2013. Biochem Biophys Res Commun. 6:1714. PubMed
Product Citations
  1. Chung H et al. 2018. Cell. 172(4):811-824 . PubMed
  2. Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed
  3. Qin H, et al. 2023. Nat Commun. 5178:14. PubMed
  4. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  5. Aomatsu M, et al. 2013. Biochem Biophys Res Commun. 6:1714. PubMed
  6. Hackel A, et al. 2021. Eur J Immunol. 51:319. PubMed
  7. Li N,et al. 2017. Cancer Immunol Immunother.. 10.1007/s00262-017-2086-8. PubMed
  8. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
RRID
AB_2121590 (BioLegend Cat. No. 308703)
AB_2121590 (BioLegend Cat. No. 308704)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution

Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts

Function
Activates Jak1, Jak2, STAT1, host defense
Ligand/Receptor
IFN-γ
Cell Type
B cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Calderon J, et al. 1988. P. Natl. Acad. Sci. USA 85:4837.
2. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
3. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

Gene ID
3459 View all products for this Gene ID
UniProt
View information about CD119 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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