- Clone
- QA19A47 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LPS receptor
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood monocytes were stained with anti-human CD14 recombinant (clone QA19A47) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 366803 | 25 tests | $117.00 | |||
| 366804 | 100 tests | $256.00 | |||
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This clone binds to CD14 in human.
- RRID
-
AB_2892445 (BioLegend Cat. No. 366803)
AB_2892445 (BioLegend Cat. No. 366804)
Antigen Details
- Structure
- Glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434. - Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Other Formats
View All CD14 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD14 Recombinant Antibody | QA19A47 | FC |
| FITC anti-human CD14 Recombinant Antibody | QA19A47 | FC |
| PE anti-human CD14 Recombinant Antibody | QA19A47 | FC |
| APC anti-human CD14 Recombinant Antibody | QA19A47 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD14 Recombinant Antibody
Human peripheral blood monocytes were stained with purified ... -
FITC anti-human CD14 Recombinant Antibody
Human peripheral blood monocytes were stained with anti-huma... -
PE anti-human CD14 Recombinant Antibody
Human peripheral blood monocytes were stained with anti-huma... -
APC anti-human CD14 Recombinant Antibody
Human peripheral blood monocytes were stained with anti-huma...