- Clone
- W18182A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
- Isotype
- Rat IgG2a, κ
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Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) APC and anti-human CD16 (clone W18182A) PE (left) or rat IgG2a, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 390905 | 25 tests | $105.00 | |||
| 390906 | 100 tests | $235.00 | |||
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD16 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone W18182A does not block the binding of clones 3G8, B73.1, and W18182B on target cells.
- RRID
-
AB_3662289 (BioLegend Cat. No. 390905)
AB_3662289 (BioLegend Cat. No. 390906)
Antigen Details
- Structure
- Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
- Distribution
-
Expressed on natural killer cells, activated monocytes, macrophages, neutrophils and a subset of T cells
- Function
- Low affinity IgG Fc receptor
- Ligand/Receptor
- IgG Fc (Fcγ)
- Cell Type
- Granulocytes, Neutrophils, NK cells
- Biology Area
- Adaptive Immunity, Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Schubert J, et al. 1989. Oxford University Press. pp 711.
- Ravetch JV and Bolland S. 2001. Annu Rev Immunol. 19:275-90.
- Gene ID
- 2214 View all products for this Gene ID 2215 View all products for this Gene ID
- UniProt
- View information about CD16 on UniProt.org
Other Formats
View All CD16 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD16 | W18182A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD16
Human peripheral blood lymphocytes were stained with anti-hu...