- Clone
- QA18A13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcγRIII, Fc gamma receptor, Fc gamma receptor 3
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with CD56 APC and anti-human CD16 recombinant (clone QA18A13) PE (left) or mouse IgG1, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 325903 | 25 tests | $117.00 | |||
| 325904 | 100 tests | $238.00 | |||
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2894473 (BioLegend Cat. No. 325903)
AB_2894473 (BioLegend Cat. No. 325904)
Antigen Details
- Structure
- Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
- Function
- Low affinity IgG Fc receptor, phagocytosis, ADCC
- Ligand/Receptor
- Aggregated IgG, IgG-antigen complex
- Antigen References
-
- Rataj, et al. 2019. Br J Cancer. 120:79-87.
- Gene ID
- 2214 View all products for this Gene ID
- UniProt
- View information about CD16 on UniProt.org
Other Formats
View All CD16 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD16 Recombinant Antibody | QA18A13 | FC |
| PE anti-human CD16 Recombinant Antibody | QA18A13 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD16 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD56 AP... -
PE anti-human CD16 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD56 AP...