PE anti-human CD172a (SIRPα) Antibody

Pricing & Availability
Clone
15-414 (See other available formats)
Regulatory Status
RUO
Other Names
BIT, MFR, P84, MYD-1, SHPS1, PTPNS1, CD172 antigen-like family member A
Isotype
Mouse IgG2a, κ
15-414_PE_CD172a_Antibody_021317
Human peripheral blood monocytes were stained with CD172a (clone 15-414) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 15-414_PE_CD172a_Antibody_021317
    Human peripheral blood monocytes were stained with CD172a (clone 15-414) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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372103 25 tests $146.00
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372104 100 tests $317.00
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Description

CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG1a cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Majdic O, et al. 1981. Blood. 58:1127.
Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Schoeppe R, et al. 2023. Front Oncol. 13:1120194. PubMed
  3. Yu PC, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
RRID
AB_2650860 (BioLegend Cat. No. 372103)
AB_2650860 (BioLegend Cat. No. 372104)

Antigen Details

Structure
Type I transmembrane protein, 85-90 kD, one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, two ITIM motifs and a proline-rich region in the cytoplasmic tail.
Distribution

Monocytes, macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells.

Function
Negative regulation of receptor tyrosine kinase-coupled signaling, mediates macrophage multinucleation, involved in neuronal survival.
Ligand/Receptor
CD47, SP-A, and SP-D.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Cell Biology, Immunology, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Lv Z, et al. 2015. J. Immunol. 195:661.
2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024.
3. Zen K, et al. 2013. Nat. Commun. 4:2436.
4. Baba N, et al. 2013. J. Exp. Med. 210:1251.
5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457.

Gene ID
140885 View all products for this Gene ID
UniProt
View information about CD172alpha on UniProt.org
Go To Top Version: 1    Revision Date: 02/13/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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