PE anti-human CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
11A8 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1, TLiSA1
Isotype
Mouse IgG1, κ
11A8_PE_CD226_DNAM-1_Antibody_1_081117
Human peripheral blood lymphocytes were stained with CD3 Brilliant Violet 421™ and CD226 (clone 11A8) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 11A8_PE_CD226_DNAM-1_Antibody_1_081117
    Human peripheral blood lymphocytes were stained with CD3 Brilliant Violet 421™ and CD226 (clone 11A8) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 11A8_PE_CD226_DNAM-1_Antibody_2_081117
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Cat # Size Price Quantity Check Availability
338305 25 tests $122.00
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338306 100 tests $226.00
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Description

DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: block NK cell-mediated lysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Fuchs A, et al. 2004. J. Immunol. 172:3994.
Product Citations
  1. Sano Y, et al. 2023. Anticancer Res. 43:547. PubMed
  2. Erbe AK, et al. 2023. J Immunother Cancer. 11:. PubMed
  3. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  4. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  5. Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
  6. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  7. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  8. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  9. Gilchrist JJ, et al. 2022. Nat Commun. 13:4073. PubMed
  10. Damele L, et al. 2021. Cancers (Basel). 13:. PubMed
  11. Rosskopf S, et al. 2018. Oncotarget. 9:17608. PubMed
RRID
AB_2259834 (BioLegend Cat. No. 338305)
AB_2259834 (BioLegend Cat. No. 338306)

Antigen Details

Structure
Ig domain containing adhesion molecule
Distribution

Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells

Function
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
Interaction
Interacts with LFA-1.
Ligand/Receptor
PVR (CD155) and Nectin-2 (CD112)
Cell Type
B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Shibuya A et al. 1996. Immunity 4:573.
2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533.
3. Pende D et al. 2005. Mol. Immunol. 42:463.

Gene ID
10666 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 2    Revision Date: 08/11/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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