- Clone
- W18100A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-21 Receptor, novel interleukin receptor (NILR)
- Isotype
- Rat IgG2a, κ
-
Human peripheral blood lymphocytes were stained with anti-human CD19 Alexa Fluor® 488 and anti-human CD360 (IL-21R) (clone W18100A) PE (left) or rat IgG2a, ĸ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 376103 | 25 tests | $141.00 | |||
| 376104 | 100 tests | $311.00 | |||
The human interleukin 21 receptor (IL-21R), is a single pass type I membrane protein and a member of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibits the greatest extracellular homology to the IL-2R beta subunit, i.e., contains one copy of the WSXWS-containing cytokine-binding domain. Intracellular domains of IL-21R include the Box 1 and Box 2 elements which are similar to the IL-9R intracellular region. Upon binding IL-21, the IL-21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Stat signaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is a potent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly the inflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Extracellular domain of hIL-21R
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2890827 (BioLegend Cat. No. 376103)
AB_2890827 (BioLegend Cat. No. 376104)
Antigen Details
- Structure
- Single pass type I membrane protein, extracellular WSXWS-containing cytokine-binding domain, intracellular Box1 and Box2 domains
- Distribution
-
B cells, various levels on NK cells, T cells, NKT cells, B cells, DCs, macrophages, non-hematopoietic cells, including keratinocytes and fibroblasts
- Ligand/Receptor
- IL-21
- Antigen References
-
1. De Leur, et al. 2017. Front Immunol. 8:306.
2. Cheekatla, et al. 2017. J Immunol. 199, 2815-2822
3. Zheng, et al. 2018. Oncoimmunology. 7(12) - Gene ID
- 50615 View all products for this Gene ID
- UniProt
- View information about CD360 on UniProt.org
Other Formats
View All CD360 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| APC anti-human CD360 (IL-21R) | W18100A | FC |
| PE anti-human CD360 (IL-21R) Antibody | W18100A | FC |
| Purified anti-human CD360 (IL-21R) Antibody | W18100A | FC |
| Brilliant Violet 421™ anti-human CD360 (IL-21R) | W18100A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD360 (IL-21R)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD360 (IL-21R) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Purified anti-human CD360 (IL-21R) Antibody
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 421™ anti-human CD360 (IL-21R)
Human peripheral blood lymphocytes were stained with anti-hu...