- Clone
- QA18A35 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T4/Leu-3
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were stained with CD3 APC and CD4 (clone QA18A35) PE (left) or mouse IgG1, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 302403 | 25 tests | $73.00 | |||
| 302404 | 100 tests | $159.00 | |||
CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Proprietary
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2860776 (BioLegend Cat. No. 302403)
AB_2860776 (BioLegend Cat. No. 302404)
Antigen Details
- Structure
- 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
- Distribution
-
Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
- Interaction
- Part of the TCR/CD3 complex
- Ligand/Receptor
- β2 domain from the MHC class II
- Cell Type
- Monocytes, NKT cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
- Vignali DA. 2010. J. Immunol. 184:5933.
- Zhou L, et al. 2009. Immunity 30:646.
- Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
- Zhu J and Paul WE. 2008. Blood 112:1557.
- Gene ID
- 920 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
Other Formats
View All CD4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD4 Recombinant Antibody | QA18A35 | FC |
| Purified anti-human CD4 Recombinant Antibody | QA18A35 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD4 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
Purified anti-human CD4 Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie...