- Clone
- QA18A47 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BP50, TNFRSF5
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD40 recombinant (clone QA18A47) PE (left) or mouse IgG1, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 325805 | 25 tests | $105.00 | |||
| 325806 | 100 tests | $225.00 | |||
CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The QA18A46 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone QA18A47 can completely block the binding of clone 5C3 on target cells.
- RRID
-
AB_2924535 (BioLegend Cat. No. 325805)
AB_2924535 (BioLegend Cat. No. 325806)
Antigen Details
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
-
B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Interaction
- TRAF signaling molecules
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591. - Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Other Formats
View All CD40 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD40 Recombinant Antibody | QA18A47 | FC |
| APC anti-human CD40 Recombinant Antibody | QA18A47 | FC |
| PE anti-human CD40 Recombinant Antibody | QA18A47 | FC |
| PE/Cyanine7 anti-human CD40 Recombinant Antibody | QA18A47 | FC |
| FITC anti-human CD40 Recombinant Antibody | QA18A47 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD40 Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD40 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD40 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD40 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD40 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...