PE anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
a-HI100_PE_011812
Human peripheral blood lymphocytes were stained with CD45RA (clone HI100) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • a-HI100_PE_011812
    Human peripheral blood lymphocytes were stained with CD45RA (clone HI100) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • b-HI100_PE_CD45RA_Antibody_04292022
    Pre-lysed human blood leukocytes were stained with CD45RA (clone HI100) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
  • c-HI100_PE_CD45RA_Antibody_3_101024
    Multiplexed IHC staining of PE anti-CD45RA (clone HI100) on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the Cellscape™. The tissue was iteratively stained with PE anti-CD45RA (clone HI100, green) and Alexa Fluor® 488 anti-CD3 (red) for one hour at room temperature. Nuclei were counterstained with DAPI. Images were captured with a 20X objective. Scale bar: 50 µm
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
304107 25 tests $44.00
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304108 100 tests $105.00
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Community Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Additional Product Notes

For the use of this antibody in spatial biology (SB), we have partnered with Bruker Spatial Biology Biosciences for demonstration of this antibody on their next-generation ChipCytometry instrument called the CellScape™. The CellScape platform is an end-to-end solution for highly multiplexed spatial omics. Combining an advanced, purpose-built imaging system with easy-to-use fluidics for walk-away automation, the CellScape system will accelerate your exploration into the rapidly evolving field of spatial biology. More information on the the Bruker Spatial Biology CellScape and a complete list of our antibodies that have been demonstrated on the instrument can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  2. Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed
  3. Gfeller D, et al. 2023. Cell Syst. 14:72. PubMed
  4. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  5. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  6. Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
  7. Nagle VL, et al. 2022. Mol Cancer Ther. 21:658. PubMed
  8. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  9. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  10. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  11. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
  12. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  13. Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
  14. Zhang M, et al. 2020. J Cell Mol Med. . PubMed
  15. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  16. Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed
  17. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  18. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  19. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  20. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  21. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  22. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  23. Hale M, et al. 2017. Mol Ther Methods Clin Dev. 0.3. PubMed
  24. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  25. Hwang HJ, et al. 2021. Immun Inflamm Dis. 9:274. PubMed
  26. Kim ML, et al. 2021. iScience. 24:103509. PubMed
  27. Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
RRID
AB_314411 (BioLegend Cat. No. 304107)
AB_314411 (BioLegend Cat. No. 304108)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Other Formats

View All CD45RA Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45RA HI100 FC
Biotin anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
PE anti-human CD45RA HI100 FC,SB
PE/Cyanine5 anti-human CD45RA HI100 FC
Purified anti-human CD45RA HI100 FC,CyTOF®,ICC,IHC-P,IHC-F,SB
Alexa Fluor® 488 anti-human CD45RA HI100 FC
Alexa Fluor® 647 anti-human CD45RA HI100 FC,IHC-P
Pacific Blue™ anti-human CD45RA HI100 FC
Alexa Fluor® 700 anti-human CD45RA HI100 FC
PerCP/Cyanine5.5 anti-human CD45RA HI100 FC,SB
PE/Cyanine7 anti-human CD45RA HI100 FC
APC/Cyanine7 anti-human CD45RA HI100 FC
Brilliant Violet 421™ anti-human CD45RA HI100 FC,IHC-P
Brilliant Violet 570™ anti-human CD45RA HI100 FC
Brilliant Violet 605™ anti-human CD45RA HI100 FC
Brilliant Violet 650™ anti-human CD45RA HI100 FC
Brilliant Violet 711™ anti-human CD45RA HI100 FC
Brilliant Violet 785™ anti-human CD45RA HI100 FC
Brilliant Violet 510™ anti-human CD45RA HI100 FC
Purified anti-human CD45RA (Maxpar® Ready) HI100 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
PerCP anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
TotalSeq™-A0063 anti-human CD45RA HI100 PG
Alexa Fluor® 594 anti-human CD45RA HI100 IHC-P
TotalSeq™-B0063 anti-human CD45RA HI100 PG
TotalSeq™-C0063 anti-human CD45RA HI100 PG
Brilliant Violet 750™ anti-human CD45RA HI100 FC
Spark NIR™ 685 anti-human CD45RA HI100 FC
APC anti-human CD45RA HI100 FC
PE/Fire™ 640 anti-human CD45RA HI100 FC
PE/Fire™ 700 anti-human CD45RA Antibody HI100 FC
Spark YG™ 581 anti-human CD45RA HI100 FC
TotalSeq™-D0063 anti-human CD45RA HI100 PG
Spark Violet™ 423 anti-human CD45RA HI100 FC,IHC-P
GMP FITC anti-human CD45RA HI100 FC
PE/Cyanine7 anti-human CD45RA HI100 FC
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark UV™ 387 anti-human CD45RA HI100 FC
GMP APC anti-human CD45RA HI100 FC
TotalSeq™-Bn0063 anti-human CD45RA HI100 SB
Spark Blue™ 550 anti-human CD45RA HI100 FC
GMP PE/Dazzle™ 594 anti-human CD45RA HI100 FC
GMP APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark PLUS UV395™ anti-human CD45RA HI100 FC
Spark Red™ 718 anti-human CD45RA HI100 FC
APC/Fire™ 810 anti-human CD45RA HI100 FC
PE/Fire™ 810 anti-human CD45RA HI100 FC
Spark Blue™ 574 anti-human CD45RA (Flexi-Fluor™) HI100 FC
Go To Top Version: 3    Revision Date: 10/10/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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