PE anti-human CD54 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of lymphoctes interaction¹. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322721 & 322722).

Application References

(PubMed link indicates BioLegend citation)

1. Evans HG, et al. 2009. Proc. Natl. Acad. Sci. USA. 106:6232. (Block) PubMed

Product Citations

1. Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed
2. Mirando AC, et al. 2020. Int J Mol Sci. 21:00. PubMed
3. Compté N, et al. 2013. PLoS One. 8:65325. PubMed
4. Buravkova LB et al. 2018. Journal of cellular biochemistry. 119(3):2875-2885 . PubMed
5. Elena R Andreeva et al. 2017. Journal of cellular physiology. 233(2):1535-1547 . PubMed
6. D'Agostino G, et al. 2018. Br J Anaesth. 121:962. PubMed
7. Chalubinski M, et al. 2021. APMIS. 129:143. PubMed
8. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
9. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
10. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
11. Gorzelak–Pabiś P, et al. 2017. Adv Clin Exp Med. 26:461. PubMed
12. Zou M, et al. 2020. Genes (Basel). 11:. PubMed

RRID

AB_535980 (BioLegend Cat. No. 322707)
AB_535980 (BioLegend Cat. No. 322708)

Antigen Details

Structure

Type I membrane protein, Ig superfamily, 85-110 kD

Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.

Function

Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.

Ligand/Receptor

CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen

Cell Type

B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells

Biology Area

Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Voraberger G, et al.. 1991 J. Immunol. 147:2777.
2. Staunton DE, et al.. 1988. Cell 52:925.
3. Greve JM, et al.. 1989. Cell 56:839.

Gene ID

3383 View all products for this Gene ID

UniProt

View information about CD54 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats